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macconkey agar medium  (Thermo Fisher)


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    Structured Review

    Thermo Fisher macconkey agar medium
    Macconkey Agar Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/macconkey+agar+medium/AGAR/pm41600700-81-10-13
    Average 99 stars, based on 1 article reviews
    macconkey agar medium - by Bioz Stars, 2026-09
    99/100 stars

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    other:

    Article Title: Investigation of plasma parameters, distributions, and optical emission for the anti-microbial performance of non-woven fabric under direct current glow discharge
    Article Snippet: Finally, we spread E. coli onto a series of Petri dishes containing MacConkey agar medium ( Oxoid Australia, Adelaide, SA ) to assess the results [ ].

    Article Title: Enhancing the Production of Eco-Friendly Silk Fabrics through the Application of Nonthermal Plasma Wettability Techniques
    Article Snippet: Finally, we spread E. coli onto a series of Petri dishes containing MacConkey agar medium (Oxoid Australia, Adelaide, SA) to evaluate the results.

    Article Title: Decoding MexB efflux pump genes: structural, molecular, and phylogenetic analysis of multidrug-resistant and extensively drug-resistant Pseudomonas aeruginosa
    Article Snippet: The collected samples were grown on Blood agar (Oxoid, UK) and MacConkey agar medium (Oxoid, UK).

    Incubation:

    Article Title: Antimicrobial Activity of Bioactive Peptides on Resistant Enterobacteriaceae and the Viability of Giardia duodenalis Cysts Isolated from Healthy Dogs.
    Article Snippet: In detail, each sample was placed in preenrichment broth (peptone water; PW; Oxoid, Rodano, Italy) and incubated aerobically at 37 ◦C for 24 h. Subsequently, the samples were streaked onto selective and differential media. .. An aliquot of broth culture (10 μL) was seeded onto MacConkey Agar medium (Oxoid, Rodano, Italy) and incubated at 37 ◦C for 24 h. All colonies were subjected to morphological recognition, oxidase and catalase tests, and streaked onto chromogenic media, such as Tryptone Bile X-Gluc agar (TBX; Oxoid, Rodano, Italy) at 37 ◦C for 24 h. In addition, bacterial identification was conducted by assessing the growth status on CHROMagar orientation medium [35] using the API 20E kit (SYSMEX bioMérieux Co., Ltd., Tokyo, Japan) and confirmed by Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight (MALDI-TOF) analysis with the Bruker MALDI Bio-typer system (Bruker Daltonics, Bremen, Germany). https://doi.org/10.3390/vetsci13010044 ..

    Article Title: Presence of Resistant Enterobacteriaceae in Poultry and Synanthropic Birds of an Urban Context of Social Farming in Southern Italy.
    Article Snippet: Each swab sample was placed in Peptone Water (Oxoid, Milano, Italy), incubated aerobically at 37 ◦C for 24 h, and then streaked on selective and differential media. .. In detail, an aliquot of culture broth (10 μL) was streaked on MacConkey Agar medium (Oxoid, Milano, Italy) and incubated at 37 ◦C for 24 h. All colonies were subjected to morphological examination, oxidase and catalase tests and streaked on chromogenic media, specifically Tryptone Bile X-Gluc agar (Oxoid, Milano, Italy) and Brilliance Salmonella Agar (Oxoid, Milano, Italy), which were incubated at 42 ◦C for 24 h. The obtained colonies were cryopreserved in Brain Heart Infusion broth (Oxoid, Milano, Italy) supplemented with glycerol (20%) for subsequent identification by Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight (MALDI-TOF). .. For isolation of Salmonella spp., the starting samples were enriched in selective Rappaport Vassiliadis broth (Oxoid, Milano, Italy) and incubated at 42 ◦C for 24 h. Subsequently, the broth cultures were streaked on Brilliant Green medium (Oxoid, Milano, Italy) and on Xylose Lysine Desoxycholate (Liofilchem Ltd., Teramo, Italy) Agar, incubated at 37 ◦C for 24 h. Suspect colonies, drop-shaped and not fermenting lactose, were subjected to oxidase tests and cryopreserved for identification by MALDI-TOF as previously described.

    Article Title: Presence of Resistant Enterobacteriaceae in Poultry and Synanthropic Birds of an Urban Context of Social Farming in Southern Italy
    Article Snippet: Each swab sample was placed in Peptone Water (Oxoid, Milano, Italy), incubated aerobically at 37 °C for 24 h, and then streaked on selective and differential media. .. In detail, an aliquot of culture broth (10 μL) was streaked on MacConkey Agar medium (Oxoid, Milano, Italy) and incubated at 37 °C for 24 h. All colonies were subjected to morphological examination, oxidase and catalase tests and streaked on chromogenic media, specifically Tryptone Bile X-Gluc agar (Oxoid, Milano, Italy) and Brilliance Salmonella Agar (Oxoid, Milano, Italy), which were incubated at 42 °C for 24 h. The obtained colonies were cryopreserved in Brain Heart Infusion broth (Oxoid, Milano, Italy) supplemented with glycerol (20%) for subsequent identification by Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight (MALDI-TOF). .. For isolation of Salmonella spp., the starting samples were enriched in selective Rappaport Vassiliadis broth (Oxoid, Milano, Italy) and incubated at 42 °C for 24 h. Subsequently, the broth cultures were streaked on Brilliant Green medium (Oxoid, Milano, Italy) and on Xylose Lysine Desoxycholate (Liofilchem Ltd., Teramo, Italy) Agar, incubated at 37 °C for 24 h. Suspect colonies, drop-shaped and not fermenting lactose, were subjected to oxidase tests and cryopreserved for identification by MALDI-TOF as previously described.

    Article Title: Antimicrobial Activity of Bioactive Peptides on Resistant Enterobacteriaceae and the Viability of Giardia duodenalis Cysts Isolated from Healthy Dogs
    Article Snippet: In detail, each sample was placed in pre-enrichment broth (peptone water; PW; Oxoid, Rodano, Italy) and incubated aerobically at 37 °C for 24 h. Subsequently, the samples were streaked onto selective and differential media. .. An aliquot of broth culture (10 μL) was seeded onto MacConkey Agar medium (Oxoid, Rodano, Italy) and incubated at 37 °C for 24 h. All colonies were subjected to morphological recognition, oxidase and catalase tests, and streaked onto chromogenic media, such as Tryptone Bile X-Gluc agar (TBX; Oxoid, Rodano, Italy) at 37 °C for 24 h. In addition, bacterial identification was conducted by assessing the growth status on CHROMagar orientation medium [ ] using the API 20E kit (SYSMEX bioMérieux Co., Ltd., Tokyo, Japan) and confirmed by Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight (MALDI-TOF) analysis with the Bruker MALDI Bio-typer system (Bruker Daltonics, Bremen, Germany). ..

    Cell Culture:




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    Isolation and identification of bacteria. (a) Growth of pure colonies of the six gut bacteria as follows: B1 and B2 in bacterial growth media, BHI1 and BHI2 in brain heart infusion, M1 and M2 in <t>MacConkey</t> media. (b) PCR amplification with 16S rRNA genes of the six isolates. The bands of high intensity correspond to positive results with positive bands at ~1500 bp of the six bacterial isolates (B1, B2, BHI1, BHI2, M1, M2). L: 1 kb DNA ladder, PC: positive control (16s rRNA fragments of Escherichia coli ATCC 8739), NC: negative control (nuclease free water).
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    Isolation and identification of bacteria. (a) Growth of pure colonies of the six gut bacteria as follows: B1 and B2 in bacterial growth media, BHI1 and BHI2 in brain heart infusion, M1 and M2 in MacConkey media. (b) PCR amplification with 16S rRNA genes of the six isolates. The bands of high intensity correspond to positive results with positive bands at ~1500 bp of the six bacterial isolates (B1, B2, BHI1, BHI2, M1, M2). L: 1 kb DNA ladder, PC: positive control (16s rRNA fragments of Escherichia coli ATCC 8739), NC: negative control (nuclease free water).

    Journal: Gut Microbes Reports

    Article Title: The living medicine inside us: in vitro therapeutic prospects of human gut bacteria

    doi: 10.1080/29933935.2025.2480093

    Figure Lengend Snippet: Isolation and identification of bacteria. (a) Growth of pure colonies of the six gut bacteria as follows: B1 and B2 in bacterial growth media, BHI1 and BHI2 in brain heart infusion, M1 and M2 in MacConkey media. (b) PCR amplification with 16S rRNA genes of the six isolates. The bands of high intensity correspond to positive results with positive bands at ~1500 bp of the six bacterial isolates (B1, B2, BHI1, BHI2, M1, M2). L: 1 kb DNA ladder, PC: positive control (16s rRNA fragments of Escherichia coli ATCC 8739), NC: negative control (nuclease free water).

    Article Snippet: These are Bacterial Growth Medium (BGM), Brain Heart Infusion (BHI) Medium (Condalab, Madrid, Spain) and MacConkey Agar Medium (Condalab, Madrid, Spain).

    Techniques: Isolation, Bacteria, Amplification, Positive Control, Negative Control